The Sakamuro Lab

Daitoku Sakamuro, PhD Gang Zhou, PhD

Professor, Georgia Cancer Center, Department of Medicine, Medical College of Georgia

Professor, Department of Biochemistry and Molecular Biology

Professor, The Graduate School, 91麻豆

Research Summary

The Sakamuro Laboratory is interested in the mechanisms through which cancer cells acquire resistance to genotoxic stresses and serum starvation. The ultimate goal of the laboratory is to establish a strategy to enhance the therapeutic benefits of conventional DNA-damaging chemotherapy in nutrition-starved cancer cells.

Contact Us

The Daitoku Sakamuro Lab

Health Sciences Campus

1410 Laney Walker Blvd., CN-2177, 91麻豆, GA 30912

(706) 721-1018

dsakamuro@augusta.edu

Research Interests

BIN1 was originally identified as the c-MYC oncoprotein-interacting adaptor protein with tumor suppressor properties. BIN1-dependent tumor suppression is unique and attractive, because BIN1 broadly counteracts the oncogenic transformation mediated not only by c-MYC, but also human papillomavirus oncoprotein E7 and dominant negative (i.e., oncogenic) mutant of TP53. Furthermore, the highly coiled-coil domain of BIN1 is necessary and sufficient to inhibit tumor cell proliferation⁴, and heterologous expression of BIN1 induces apoptosis only in transformed cells. The BIN1 gene transcription is robustly increased following DNA damage, implying that BIN1 is involved in cellular responses to DNA damage. Consistent with this, BIN1 interacted with the DNA-repair-priming enzyme poly(ADP-ribose) polymerase 1 (PARP1) and inhibited its catalytic activity. Accordingly, BIN1 acted as an inducer of genomic instability and rendered cancer cells sensitive to chemotherapy. The Sakamuro laboratory also found that oncogenic c-MYC directly suppresses the BIN1 gene transcription in the presence of the MYC-interacting zinc finger protein 1 (MIZ1). As expected, treatments of chemoresistant cancer cells with 10058-F4 (the small molecule c-MYC inhibitor) released endogenous BIN1 expression and robustly increased the sensitivity to cisplatin. They will explore whether PARP1 is the only BIN1 target to inhibit when BIN1 increases genomic instability.

The transcription factor E2F1 induces apoptosis after DNA damage and serum starvation. In response to DNA damage, E2F1 is phosphorylated by ataxia telangiectasia-mutated (ATM) kinase to promote apoptosis. However, precisely how serum starvation stimulates E2F1-induced apoptosis was unclear. The Sakamuro laboratory found that PARP1 interacts directly with E2F1, increases E2F1 transactivation, and induces G₂/M cell-cycle arrest under optimal conditions. In contrast, inhibition of PARP1 enhanced E2F1-induced apoptosis in serum-starved cells. Interestingly, basal PARP1 activity modified E2F1 by poly(ADP-ribosyl)ation, which stabilized the interaction between E2F1 and BIN1 in the nucleus. When the BIN1鈥揈2F1 interaction was abolished by PARP1 inhibition, E2F1 continuously increased BIN1 levels. They also found that serum starvation massively reduced the E2F1 poly(ADP-ribosyl)ation. These results suggest that the release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis. In the vein of hypoxia, serum starvation generally arises in compacted cancer tissues, particularly after chemotherapy and anti-angiogenic therapy. Restoration of the sensitivity to serum-starvation-induced apoptosis may improve chemotherapeutic benefits in late-stage (i.e., chemo- and radiation-resistant) cancer cells.

Selected Publications

Sakamuro D, Prendergast GC. (1999) Oncogene.18:2942-54. Review

Elliott K, Sakamuro D, Basu A, Du W, Wunner W, Staller P, Gaubatz S, Zhang H, Prochownik E, Eilers M, Prendergast GC. (1999) Oncogene. 18:3564-73.

Kinney EL, Tanida S, Rodrigue AA, Johnson JK, Tompkins VS, Sakamuro D. (2008) J Cell Physiol. 216:621-31.

Lundgaard GL, Daniels NE, Pyndiah S, Cassimere EK, Ahmed KM, Rodrigue A, Kihara D, Post CB, Sakamuro D. (2011) J Cell Biochem. 112:2992-3001.

DuHadaway JB, Sakamuro D, Ewert DL, Prendergast GC. (2001) Cancer Res.61:3151-6.

91麻豆

Information for:

Resources